Journal: Bioengineering

Article Title: Modulation of Canine Adipose-Derived Mesenchymal Stem/Medicinal Signalling Cells with Ascorbic Acid: Effect on Proliferation and Chondrogenic Differentiation on Standard Plastic and Silk Fibroin Surfaces

doi: 10.3390/bioengineering11050513

Figure Lengend Snippet: Data on antibodies and dilutions used for flow cytometry analysis in the study.

Article Snippet: Cells were stained with the following antibodies for canine ADMSCs: allophycocyanin (APC) conjugated against CD44 (antibody clone IM7, 103012, Biolegend, San Diego, CA, USA), phycoerythrin (PE) conjugated against CD90 (antibody clone YKIX337.217, 12-5900-42, eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC) conjugated against CD29 (antibody clone MEM-101A, MA1-19566, ThermoFisher Scientific, Waltham, MA, USA) and CD34 (antibody clone 581, 60013FI, Stemcell Technologies, Cambridge, MA, USA).

Techniques: Flow Cytometry, Marker, Conjugation Assay

Journal: Bioengineering

Article Title: Modulation of Canine Adipose-Derived Mesenchymal Stem/Medicinal Signalling Cells with Ascorbic Acid: Effect on Proliferation and Chondrogenic Differentiation on Standard Plastic and Silk Fibroin Surfaces

doi: 10.3390/bioengineering11050513

Figure Lengend Snippet: Expression of cAMSC surface markers. In the upper row are histograms of the negative marker CD34-FITC (blue) and the positive markers CD29-FITC, CD44-APC, and CD90-PE. In the lower row, dot plots of the positive stem cell markers CD29, CD44, and CD90 are presented. Blue and green are cells labelled with antibodies. Images of negative control cells that were not labelled with antibodies (red) are appended to the images of the labelled (green) cells in both histograms and dot plots. In the dot plots, the percentage of negative control cells is 100% (not shown), while the percentage of labelled cells is shown in each quadrant of the dot plots.

Article Snippet: Cells were stained with the following antibodies for canine ADMSCs: allophycocyanin (APC) conjugated against CD44 (antibody clone IM7, 103012, Biolegend, San Diego, CA, USA), phycoerythrin (PE) conjugated against CD90 (antibody clone YKIX337.217, 12-5900-42, eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC) conjugated against CD29 (antibody clone MEM-101A, MA1-19566, ThermoFisher Scientific, Waltham, MA, USA) and CD34 (antibody clone 581, 60013FI, Stemcell Technologies, Cambridge, MA, USA).

Techniques: Expressing, Marker, Negative Control

Journal: AIMS Microbiology

Article Title: Deregulation of the cyclin-dependent kinase inhibitor p27 as a putative candidate for transformation in Chlamydia trachomatis infected mesenchymal stem cells

doi: 10.3934/microbiol.2023009

Figure Lengend Snippet: Representative histogram and Western blotting of the stemness markers expression in isolated MSCs. Cells isolated from fallopian tubes displayed stemness markers. The histograms in panel (B) show cells stained with anti-CD44 (FITS-green), anti CD90 (PE-yellow), and anti-CD73 (APC-red). The results were compared to unstained cells in panel (A). In panel (C), host cells were cultured and protein samples were collected at 72 h post-infection. Protein samples for the stemness markers Sox2, Nanog, Oct4, CD44, CD90, and CD73 were detected by Western blotting. β-actin was used as a loading control.

Article Snippet: The used antibodies were specific for CD44 directly conjugated with fluorescein isothiocyanate (FITC) (1:100, Mouse, BD Pharmingen, USA), CD73- allophycocyanin (APC) (1:100, mouse, BioLegend, USA) and CD90- phycoerythrin (PE) (1:100, Mouse, BD Pharmingen, USA).

Techniques: Western Blot, Expressing, Isolation, Staining, Cell Culture, Infection